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Genetically Incorporated Non-Canonical Amino Acids Methods and Protocols - ebooks1001 - 08-21-2025
![[Image: 1b4ce4949f48409f14bc76a8a22790d3.webp]](https://i125.fastpic.org/big/2025/0818/d3/1b4ce4949f48409f14bc76a8a22790d3.webp)
Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols
by Yu-Hsuan Tsai, Simon J. Elsässer
English | 2023 | ISBN: 1071632507 | 297 Pages | True ePUB | 28 MB
This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.
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